TABLE 1.
Plasmids used in transcriptional-activation studies
| Gene | Plasmid
|
|||
|---|---|---|---|---|
| Insect vector | Yeast vector | Notes | Source | |
| Reporter genes | ||||
| hb-CAT | pCZ3005 | This study | ||
| kni-CAT | pCZ3006 | This study | ||
| hb(6A)-CAT | pCZ3007 | This study | ||
| kni(6A)-CAT | pCZ3008 | This study | ||
| hb-lacZ | pMA630R | 16 | ||
| kni-lacZ | pTA123 | This study | ||
| hb(6A)-lacZ | pCZ3004 | This study | ||
| kni(6A)-lacZ | pTA170 | This study | ||
| lexAOP-lacZ | pJP167 | J. Pearlberg | ||
| hb-lacZ(2μm) | pCZ3015 | This study | ||
| kni-lacZ(2μm) | pCZ3017 | This study | ||
| hb(6A)-lacZ(2μm) | pCZ3016 | This study | ||
| kni(6A)-lacZ(2μm) | pCZ3018 | This study | ||
| Activator genes | ||||
| HA-Bcd | pFY403 | This study | ||
| HA-Ftz(Q50K) | pCZ2079 | This study | ||
| HA-Ftz(Q50K)-VP16 | pCZ2087 | This study | ||
| HA-Bcd-Ftz(Q50K)HD-VP16 | pCZ2088 | This study | ||
| HA-Ftz-BcdHD-VP16 | pCZ2089 | This study | ||
| HA-Bcd-VP16 | pCZ2090 | This study | ||
| LexA-Bcd | pCZ99 | LEU2 marker | This study | |
| LexA-Ftz(Q50K) | pCZ97 | LEU2 marker | This study | |
| LexA-Bcd-Ftz(Q50K)HD | pCZ100 | LEU2 marker | This study | |
| LexA-Ftz-BcdHD | pCZ2038 | LEU2 marker | This study | |
| Bcd-VP16 | pMA1226 | LEU2 marker | 38 | |
| Ftz(Q50K)-VP16 | pCZ2035 | LEU2 marker | This study | |
| Bcd-Ftz(Q50K)HD-VP16 | pCZ2036 | LEU2 marker | This study | |
| Ftz-BcdHD-VP16 | pCZ2037 | LEU2 marker | This study | |