TABLE 2.
Plasmids used in biochemical studies
| Gene | Plasmid | Notes | Source |
|---|---|---|---|
| Bcd site | |||
| kni enhancer | pCZ72 | This study | |
| hb enhancer | pMAX1 | 37 | |
| hb(6A) enhancer | pCZ3003 | This study | |
| For homeodomain expression | |||
| GST-BcdHD | pCZ10 | 13 | |
| GST-Ftz(Q50K)HD | pCZ57 | 13 | |
| For in vitro translation | |||
| LexA-Bcd | pCZ95 | SP6 promoter | This study |
| LexA-Ftz(Q50K) | pCZ90 | SP6 promoter | This study |
| LexA-Bcd-Ftz(Q50K)HD | pCZ96 | SP6 promoter | This study |
| LexA-Ftz-BcdHD | pCZ2029 | SP6 promoter | This study |
| Bcd | BcdTN3 | SP6 promoter | 16 |
| Ftz(Q50K) | pFY1002 | SP6 promoter | This study |
| Bcd-Ftz(Q50K)HD | pFY1005 | SP6 promoter | This study |
| Ftz-BcdHD | pCZ2034 | SP6 promoter | This study |