FIG. 1.

Suppression of the conditional lethal phenotype of ΔRPB4 by three high-copy-number suppressors. (A) YPD plates were spotted with wild-type (wt), isogenic mutant ΔRPB4 (▵4), and suppressed cells (▵4 + suppressor 1, 2, or 3) followed by incubation at the temperatures indicated. (B) All three suppressor genes are transcribed at high levels in suppressed cells. RNA was prepared from the strains indicated and hybridized to radioactively labeled DNA corresponding to the coding region of each suppressor. Equivalent levels of total RNA were loaded in each lane.