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Suppression by RPB7 and Sro9p alters expression of certain heat shock genes. Total RNA was prepared from cells exposed to the indicated conditions and was hybridized to HSP26, SSA1, or SSA3 DNA. U3 (RNA polymerase II transcribed snRNA) was included as a loading control, test transcripts were normalized to U3, and activation levels were graphed relative to the activated wild-type control.
