FIG. 6.

Some suppressors correct activation defects at inducible genes. (A and B) Northern blot analysis of GAL1 and PHO5 transcripts before (−) and after (+) activation. (C) Northern blot analysis of INO1 transcripts under activating conditions only (no INO1 transcripts are present before activation). All RNA was prepared from cells grown at the permissive temperature (27°C). U3 (RNA polymerase II transcribed snRNA) was included as a loading control, test transcripts were normalized to U3, and activation levels were graphed relative to the activated wild-type control.