TABLE 1.

Yeast strains and plasmids used in this study

Strain or plasmid	Genotype or description
Strains
N114	MATα his3Δ200 leu2-3 leu2-112 ura3-52
WY-4	MATα RPB4Δ1::HIS3 his3Δ200 leu2-3 leu2-112 ura3-52
WY-197	MATα RPB4Δ1::HIS3 his3Δ200 leu2-3 leu2-112 ura3-52 pNW247
WY-198	MATα RPB4Δ1::HIS3 his3Δ200 leu2-3 leu2-112 ura3-52 pNW251
WY-199	MATα RPB4Δ1::HIS3 his3Δ200 leu2-3 leu2-112 ura3-52 pRP721
WY-200	MATα RPB4Δ1::HIS3 his3Δ200 leu2-3 leu2-112 ura3-52 pNW249
Plasmids
pNW247	1.8-kb EcoRI/XhoI SRO9-containing fragment in YEplac181
pNW248	SRO9-coding region with PCR-constructed EcoRI ends in pGEX-4T-1
pNW249	pNW247 lacking region encoding amino acids 297–338 of SRO9
pNW250	Nsp1p-coding region with PCR-constructed BamHI ends in pGEX-2TK
pNW251	3.5-kb NSP1-containing BamHI fragment in YEplac181
pRP716	RPB7-coding region with PCR-constructed BamHI (5′)-EcoRI (3′) ends in pGEX-2T
pRP721	1.5-kb RPB7-containing PCR fragment with ∼700-bp 5′ (BamHI added) and ∼300-bp 3′ (SalI added) DNA in YEplac181
pRP731	YEplac181 containing BamHI/SalI fragment encoding RPB7 lacking amino acids 1–12
pRP732	YEplac181 containing BamHI/SalI fragment encoding RPB7 lacking amino acids 1–22
pRP733	YEplac181 containing BamHI/SalI fragment encoding RPB7 lacking amino acids 1–33
pRP734	YEplac181 containing BamHI/SalI fragment encoding RPB7 lacking amino acids 1–49
pRP735	YEplac181 containing BamHI/SalI fragment encoding RPB7 lacking amino acids 157–171
pRP736	YEplac181 containing BamHI/SalI fragment encoding RPB7 lacking amino acids 143–171
pRP737	YEplac181 containing BamHI/SalI fragment encoding RPB7 lacking amino acids 125–171
pRP738	YEplac181 containing BamHI/SalI fragment encoding RPB7 lacking amino acids 109–171