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. 2021 Nov 22;22(12):1577–1589. doi: 10.1038/s41590-021-01059-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — Related to Fig. 1. Dot plot depicting the expression of all surface markers by cell type. Color indicates mean normalized expression, point size indicates the fraction of cells positive for the marker. Automatic thresholding was used to identify positive cells, see Methods, section ‘Thresholding of surface marker expression’ for details. The panel on the right depicts the fraction of total reads obtained for each marker as a proxy for absolute expression levels. Bottom panel illustrates the distribution of CD34 + expression across populations, similar plots can be generated for any marker using the Abseq App.