Figure 4. Mouse Pre-piRNA Trimming in Testis Lysate.
MIWI piRISC loaded with a 2′-hydroxyl, 5′-32P-radiolabeled, synthetic, 45-nt pre-piRNA was incubated in total lysate or subcellular fractions of C57BL/6 or Pnldc1em1/em1 testes.
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