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. Author manuscript; available in PMC: 2022 Dec 2.
Published in final edited form as: Mol Cell. 2021 Sep 29:S1097-2765(21)00746-2. doi: 10.1016/j.molcel.2021.09.012

Figure 5. Guanine or Uridine Tracts Destabilize Untrimmed pre-piRNAs in Testis Lysate and In Vivo.

Figure 5.

(A) MIWI piRISC loaded with a 2′-O-methyl, 5′-32P-radiolabeled, synthetic, 45-nt pre-piRNA was incubated in Pnldc1em1/em1 testis lysate. A representative experiment is shown, the lifetime is mean ± SD (n = 3).

(B) Frequency of 5mers in all pre-piRNAs or in unstable pre-piRNAs in FACS-purified Pnldc1em1/em1 mouse primary spermatocytes. A representative experiment is shown.