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. Author manuscript; available in PMC: 2022 Dec 2.
Published in final edited form as: Mol Cell. 2021 Sep 29:S1097-2765(21)00746-2. doi: 10.1016/j.molcel.2021.09.012

Figure 6. In Mice, a Decrease in piRNA Abundance Causes a Corresponding Reduction in Target RNA Cleavage.

Figure 6.

(A) Strategy to identify piRNA target RNAs by sequencing 5′ monophosphorylated RNAs.

(B) The change in steady-state abundance of putative 3′ cleavage products produced by piRNA-guided MILI- or MIWI-catalyzed cleavage. Targets were required to pair contiguously with piRNA nucleotides g2–g14. Data are from FACS-purified primary spermatocytes. Only piRNAs whose abundance was ≥ 50 molecules per C57BL/6 primary spermatocyte were analyzed. P values were calculated using a two-tailed KS test.