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. Author manuscript; available in PMC: 2022 Dec 2.
Published in final edited form as: Cell Stem Cell. 2021 Sep 16:S1934-5909(21)00376-3. doi: 10.1016/j.stem.2021.08.015

Figure 6. Human microglia attenuate type I Interferon response and decrease double-stranded DNA breaks in radial glia.

Figure 6.

A. Individual differentially expressed genes in organoids with and without microglia driving pathway analysis from Fig. 5D. B. Immunofluorescence validation of IFITM3, Interferon Induced Transmembrane Protein 3, in rosettes from organoids with and without microglia. IFITM3 fluorescence signal was averaged across the rosette area (outlined in white). C. IFITM3 has a small but statistically significant decrease in rosettes from organoids with microglia. p-value <0.05. N are individual rosettes for each condition, quantified from sections from three independent organoids per condition. D. An example of a rosette labeled with Sox2 for radial glial cells and a marker for double-stranded DNA breaks, pH2AX. A zoomed in window is an example of cell nuclei with pH2AX puncta. E. Averaged number of pH2AX puncta per cell nucleus. Cells nuclei with 2–30 pH2AX-positive puncta were used for analysis. p-value < 0.001. F. Distribution of pH2AX number of puncta between conditions. Sox2-positive nuclei from microglia-transplanted organoids had a larger proportion of cells with fewer number of puncta. Organoids without microglia had consistently higher proportion of cells with the number of puncta over 10.

See also Figure S7.