Fig. 1. Neutralizing antibody responses against SARS-CoV-2 VOC in mice, rabbits, and cynomolgus macaques after one, two, and three vaccinations with Wuhan-based Spike vaccines.

a Longitudinal analysis of pseudovirus neutralization of B.1, and VOC Alpha, Beta, and Gamma in mice (left), and of B.1, Alpha, Beta, Gamma, and Delta in rabbits (middle) and macaques (right). Datapoints represent mean ID₅₀’s, error bars indicate SD. b Paired comparisons of B.1 and Beta neutralization 2 weeks after the second and third vaccination in mice (left), rabbits (middle), and macaques (right). c Paired comparisons of Beta neutralization 2 weeks after the third vaccination and B.1. neutralization after the second vaccination in mice (left), rabbits (middle), and macaques (right). Mice and rabbits groups immunized with SARS-CoV-2 S-I53-50NP and SARS-CoV-2 S were combined for the analyses because the responses were overall quite comparable, but the individual data are given in Supplementary Tables 1 and 2. Mouse sera were pooled per group because of low serum amounts. The neutralization titers between variants were compared using the Wilcoxon test (*P < 0.05; **P < 0.01).