Table 3.

Comparison of experimental methods for the verification of SCAMs.

Assay	Principle	Feature	Readout	Note
Nonionic detergent washing	Detergents will promote the formation of monomers, thus reducing the apparent activity	Easy to operate and low cost	Enzyme inhibition	Detergent should not contribute to the readouts
Centrifugation	Centrifugation will induce the formation of pellets originating, thus changing the apparent activity	Easy to operate and can be applied to HTS platforms	Enzyme inhibition	Selectivity of non-SCAMs to tumor cells should be higher than that of normal cells
Competitive ligand−target binding experiment	SPR can identify their nonspecific aggregation-type binding mechanisms	Easy to operate and provide more detailed mechanism information	Enzyme inhibition	Compounds should be injected into the protein surface and attached to the surface of the optical biosensor
Printable hydrogel microarray	The adjustable porosity of the hydrogels allows selective transport of substrates to and from the entrapped enzyme via size selectivity	Easy to operate	Enzyme inhibition	No special requirements