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. 2021 Mar 13;11(11):3553–3566. doi: 10.1016/j.apsb.2021.03.021

Figure 2.

Figure 2

Identification of PKM2 as a direct binding target for PCA. (A) The employed drug affinity responsive target stabilization (DARTS) assay detects a marked increase in ∼60 kD band upon PCA incubation in pronase digested H9C2 cell lysates. (B) Immunoblot analysis of PKM2 in pronase-digested cell lysate (n = 3). (C) Recombinant PKM2 in pronase-digested H9C2 cell lysates. PKM2 degradation in H9C2 cell lysates (D) and in intact cells (E) (n = 3). (F) Surface plasmon resonance (SPR) binding curve fit to a Kd = 5.76 nmol/L for PCA and PKM2 (n = 3). Results are shown as mean ± SD; ∗P < 0.05, ∗∗∗P < 0.001.