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Five week-old WT, Prk–/–, Akt2–/––Ampkα2–/––Prk–/– triple knockout (TKO) mice were placed on a 45% HF or nutritionally matched LF diet for 20 weeks. (a) Representative photographs of mice (prone and supine positions) and white adipose tissues. (b) Trajectory of body weight of WT, Prk–/–, and TKO mice. (c) White adipose tissue (WAT). (d) Brown adipose tissue (BAT). (e) Spleen weight. (f) Kidney weight. Data are expressed as mean ± SEM (n = 7). ∗P < 0.05 vs. WT mice.
