Figure 4.

Metabolic properties (24-h cycle) of WT, Ampkα2^−/−, Akt2^−/−, and DKO mice fed LF or HF diet for 20 weeks. (a) O₂ consumption in DKO and WT groups. (b) O₂ consumption in Ampkα2^−/− and Akt2^−/− mice. (c) Pooled data of O₂ consumption (24 h). (d) CO₂ production in DKO and WT mice. (e) CO₂ production in Ampkα2^−/− and Akt2^−/− mice. (f) Pooled data of CO₂ production (24 h). (g) Respiratory exchange ratio (RER) in DKO and WT mice. (h) RER in Ampkα2^−/− and Akt2^−/− mice. (i) Pooled data of RER (24 h). (j) Heat production in DKO and WT mice. (k) Heat production in Ampkα2^−/− and Akt2^−/− mice. (l) Pooled data of heat production (24 h). (m) Total physical activity in DKO and WT mice. (n) Total physical activity in Ampkα2^−/− and Akt2^−/− mice. (o) Pooled data of total physical activity (24 h). Data are expressed as mean ± SEM (n = 7). ∗P < 0.05 vs. WT mice; ^#P < 0.05 vs. WT-HF mice; ^†P < 0.05 vs. DKO-HF mice.