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Effects of Ampkα2−/−, Akt2−/−, and DKO on NAFLD. (a) Hematoxylin and eosin (H&E) staining of liver cross-sections from mice fed LF or HF diet for 20 weeks. (b) Representative oil red O staining micrographs. (c) Liver weight. (d) Liver triglyceride content. (e) Serum AST levels. (f) Serum ALT levels. (g) Serum triglyceride levels. (h) Serum cholesterol levels. Data are expressed as mean ± SEM (n = 8). ∗P < 0.05 vs. WT mice; #P < 0.05 vs. WT-HF mice; †P < 0.05 vs. DKO-HF mice.
