Figure 7.

Effects of Ampkα2^−/−, Akt2^−/−, and DKO on the levels of lipid regulatory proteins in livers from mice fed LF or HF diet. (a–l) The protein levels of lipid synthesis, metabolism, and storage enzymes, including FAS (a), ACC (phosphorylated ACC and pan-ACC, pACC-to-ACC ratio, b), pan-ACC (c), CHREBP (d), SREBP1c (e), PGC-1α (f), PPARα (g), PPARγ (h), SCD-1 (i), DGAT1 (j), PEPCK (k), and G6Pase (l), were examined using Western blot analysis. Representative gel blots which depicted the levels of lipid regulatory proteins (using GAPDH or α-tubulin as loading control) were shown. Data are expressed as mean ± SEM (n = 7–10). ∗P < 0.05 vs. WT mice; ^#P < 0.05 vs. WT-HF mice; ^†P < 0.05 vs. DKO-HF mice.