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. 2021 Dec 3;12:7064. doi: 10.1038/s41467-021-27291-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Cells seeded at low density into six-well plates were cultured in the presence of increasing concentrations of cisplatin or vehicle (DMSO). Colonies arising were stained using crystal violet dye. Clonogenic survival was quantified using dye extraction. a–c Platinum response in RB1-defective (red) or RB1-normal (black) osteosarcoma and BRCA2-mutant CAPAN1 (blue). a Concentration-response curve and b scatter plots depicting AUC value comparison and c log IC50 values, deduced from the concentration-response data in a. Bars in scatter plots (b, c) depict median (±95%CI), calculated using a two-tailed Mann–Whitney test, p (b) = 0.3730^ns. Data points in a–c reflect the mean of parallel duplicate wells for n = 1 dataset. d–f RB1-normal osteosarcoma CAL72 cells transduced with lentivirus vector encoding different RB1-targeting shRNAs (shRB1-1, shRB1-2 or shRB1-3) (red), or empty vector backbone (vector), or left unmodified (unmod) (black). d Concentration-response curve, e scatter plots depicting AUC value comparison and f log IC50 values, deduced from the concentration-response data in d. Bars in scatter plots (e, f) depict median ±95%CI. Data in d depict means of parallel duplicate wells for one of n = 2 biologically independent datasets, data in e and f summarise results for n = 2 biologically independent datasets. p (e) = 0.0095, calculated using a two-tailed Mann–Whitney test, **p < 0.01. Source data are provided as a Source Data file.