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. 2021 Dec 3;6:84. doi: 10.1038/s41536-021-00194-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Plasma samples from five animals in each of the control and MSC-treated group, and three in the sham group were collected at the indicated time points, and a THBS1 and b Antithrombin III levels determined by ELISA (means ± SD). The Kruskal–Wallis test was performed (P = 0.0001) and significant differences between the groups were evaluated by post hoc Bonferroni test. c THBS1 and CD36 mRNA levels were determined in liver samples of three animals in each group at 24 h after hepatectomy. PCR products were quantified using PPIA (Peptidylprolyl Isomerase A) for normalization. Values are given as means ± SD (n = 3; statistics: one-way ANOVA) as calculated from the representative gels shown in Supplementary Fig. 2. d Immunohistochemical detection of THBS1 (arrowheads) in the liver (upper images) and lung (lower images) of sham (left), control (middle), and MSC-treated (right) pigs at 24 h after liver surgery. Images show ×20 original magnifications of representative organ tissue slices from three pigs per group. In the liver, staining is located in the portal tracts lining blood vessels and bile ducts (white arrowheads) and in the parenchyma (black arrowheads). In the lung, THBS1 is expressed in cells lining the alveolae (white arrowheads; the insets show higher magnifications (computed) of the dashed areas).