Skip to main content
. 2021 May 27;11(11):3465–3480. doi: 10.1016/j.apsb.2021.05.027

Figure 4.

Figure 4

SW33-induced apoptosis of U87 and U251 cells via mitochondrial disruption. Morphological changes in nuclei of U87 (A) and U251 (B) treated with SW33. Scale bar = 100 μm. (C) Flow cytometry using PI/Annexin V-FITC double stain showed SW33 induced apoptosis of U87 at 24 and 48 h. (D) Flow cytometry analysis using 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolocarbocyanine iodide (JC-1) stain showed that SW33 reduced mitochondrial membrane potential of U87 at 24 and 48 h. Western blotting detection of proteins associated with apoptosis in U87 cells treated with 0, 4, 8, 12 μmol/L SW33 for 24 (E) and 48 h (F). Expression of BAX was up-regulated while ratios of cleaved PARP/PARP, cleaved caspase 9/caspase 9, cleaved caspase 3/caspase 3 and expression of BCL-2 were reduced. Experiments were performed in triplicate.