FIGURE 5.

Expression of PIK3R1 is downregulated in human sarcomas, and the expression of miR‐21‐5p is upregulated in MSCs, MSC‐derived exosomes and OS cells. (A) Analysis of PIK3R1gene expressions in different subtypes of sarcomas using the Oncomine database. Box plot derived from gene expression data in the Oncomine database comparing the expressions of PIK3R1 between normal tissues and cancer tissues in different subtypes of sarcomas, pleomorphic myxofibrosarcoma, myxofibrosarcoma, myxoid/round cell liposarcoma, leiomyosarcoma and malignant fibrous histiocytoma. (B) The expression of miR‐21‐5p in MSCs, hFOB1.19 and their purified exosomes examined by RT‐qPCR. (C) The expression of miR‐21‐5p in U2OS and MG63 transfected with MSC‐derived exosomes and MGC‐GW8469 for 48 h examined by RT‐qPCR. Quantitative data from three independent experiments are shown as the mean ± SD (error bars). *p < 0.05, ***p < 0.001 (Student's t test)