Figure 1.

miR‐16‐5p targets VEGFA and inhibits its expression. (A,B) the miRNAs that could potentially be involved in the regulation of human or rat VEGFA predicted by miRNA‐gene relationship prediction websites, including DIANA, miRDB, TargetScan, miRWalk and miRSearch websites. The comparison of the predicted results was displayed by a Venn diagram. C, the targeting relationship between miR‐16‐5p and VEGFA predicted by dual‐luciferase reporter gene assay. D, Western blot analysis was performed to determine VEGFA expression in HPDCs stimulated by high glucose in the presence of miR‐16‐5p compared with that of miR‐16‐5p inhibitor. *P < .05 vs the VEGFA‐MUT group. Measurement data were expressed as mean ± standard deviation. Data between two groups were analysed using t test, and the experiment was repeated three times. HPDCs, human podocytes; miR‐16‐5p, microRNA‐16‐5p; MUT, mutant; VEGFA, vascular endothelial growth factor A