Figure 2.

High glucose stimulation inhibits the expression of miR‐16‐5p and nephrin in HPDCs, whereas promotes the expression of VEGFA. A, RT‐qPCR was employed to determine the expression of miR‐16‐5p, VEGFA and nephrin in the control, MA and HG groups. B, Western blot analysis was used to determine the protein expression of VEGFA and nephrin in each group. *P < .05 vs the control group. Measurement data were expressed as mean ± standard deviation. Multivariate analysis of variance was used for comparison among multiple groups, and the experiment was repeated 3 times. HG, high glucose; HPDCs, human podocytes; miR‐16‐5p, microRNA‐16‐5p; RT‐qPCR, reverse transcription‐quantitative polymerase chain reaction; VEGFA, vascular endothelial growth factor A