Figure 6.

Depletion of VEGFA contributes to relieved podocyte injury induced by HG. The expression of VEGFA was depleted. A, the viability of podocytes was detected by CCK‐8 assay. B, TUNEL was performed to measure the apoptosis rate of podocytes (scale bar = 25 μm). C, Western blot analysis was applied to determine the expression of nephrin, α‐SMA, Bax and Caspase‐3. D, the expression of VEGFA and relate factors MCP‐1, TGF‐β1 and TNF‐α examined by Western blot analysis. Measurement data were expressed as mean ± standard deviation. Multivariate analysis of variance was used for comparison among multiple groups, and the experiment was repeated three times. *P < .05 vs the siRNA‐NC group. CCK‐8, Cell Counting Kit‐8; HG, high glucose; MCP‐1, monocyte chemoattractant protein‐1; TGF‐β1, transforming growth factor‐β1; TNF‐α, tumour necrosis factor‐α; TUNEL, Terminal deoxynucleotidyl transferase (TdT)‐mediated 2′‐Deoxyuridine 5′‐Triphosphate (dUTP) nick‐end labelling; VEGFA, vascular endothelial growth factor A