. 2019 Sep 30;25(23):10798–10813. doi: 10.1111/jcmm.14558

Table 1.

Primer sequences for RT‐qPCR

Gene	Primer sequence
miR‐16‐5p	F: 5′‐ ATAGAATCCTTGTATTATTATGTTTGGAC‐3′
miR‐16‐5p	R: 5′‐ ATAGGATCCAAATTATACTAGCAGGA‐3′
U6	F: 5′‐ ATGACGTCTGCCTTGGAGAAC‐3′
U6	R: 5′‐ TC AGTGTGCTACGGAGTTCAG‐3′
VEGFA	F: 5′‐ GGGCTGCTGCAATGATGAA‐3′
VEGFA	R: 5′‐ TCCGCATGATCTGCATAGTGA‐3′
nephrin	F: 5′‐ AAAGGTACCAGATAAGCAGGCAGCAGGAGT‐3′
nephrin	R: 5′‐ AAACTCGAGTTGCACACCTGGCTTCGGCCT‐3′
GAPDH	F: 5′‐ AAGCTGGTCATCAATGGGAAAC‐3′
GAPDH	R: 5′‐ ACCCCATTTGATGTTAGCGG‐3′

Abbreviations: GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; miR‐16‐5p, microRNA‐16‐5p; RT‐qPCR, reverse transcription‐quantitative polymerase chain reaction; VEGFA, vascular endothelial growth factor A.