Table 3.

Values of K_{a_virus_T} calculated for SARS-CoV-2 and AAV-2 with Equation 10 using published data for K_VI and IC₅₀. The first dissociation constant for the heparin-saturated virus, K_VI, equals the dissociation constant, K_di, measured experimentally for a single SGP binding to a single heparin (see Methods for justification).

Virus	Inhibitor	IC50 (μg/cm3)	Mr (Da)	IC50 (M)	KVI (M)	aKa_virus_T (M−1)
SARS-CoV-2	UFH	b33.0	f16,000	d2.1 × 10−6	e0.73 × 10−10	3.53 × 1017
AAV-2	HS			c0.3 × 10−6	c3.4 × 10−9	1.09 × 1015

^aCalculated with [C_free] ∼ [C_total] = 8 × 10⁻¹⁴ M in Equation 10 as for human lung model (see text).

^bAverage of 25 and 41 μg/cm³ range for plaque inhibition assay of Vero E6 cells by UFH from Tree et al. (2020)

^cData for HS binding to AAV-2 from Negishi et al. (2004)

^dValue in units of M used in Equation 10 calculated as IC₅₀ in μg/cm³ converted to g/dm³ and then divided by M_r in Da.

^eTrimeric SARS-CoV-2 SGP binding to immobilized heparin (Kim et al. 2020).

^fM_r UFH (Tree et al. 2020)