Fig. 3.

WNK3 is the downstream target gene of miR-130a-3p. A The transfection efficiency was measured by qRT–PCR. B Relative expression of WNK3 after transfection with the miR-130a-3p mimic. C Top: The binding site for miR-130a-3p in the WNK3 3′-UTR. Bottom: HEK-293 T cells were transfected with the miR-130a-3p mimic/NC and luciferase reporter vector containing the WNK3 3′-UTR (WT) or its mutant construct (Mut). The WT-NC value was set to “1.0”. D Top: Relative expression of WNK3 between normal tissue and tumor tissue in LUAD. Bottom: Relative expression of WNK3 between normal tissue and tumor tissue in lung squamous cell carcinoma (LUSC). E High levels of WNK3 in NSCLC samples were related to poor OS