FIG. 7.

RNase protection analysis of su(s)wt and su(s)ΔARM1,2 RNA levels. Total RNA was isolated from adult flies reared at 25°C. Flies with one copy of a transgene contained the third chromosome balancer TM2 and a recombinant third chromosome carrying a UAS-su(s) transgene and the hs-GAL4 driver. Flies with both transgenes contained a wild-type transgene on one of the third chromosomes and a mutant transgene on the other. The transgenic flies also carry the null su(s)^R39 allele on the X chromosome and thus produce no endogenous su(s) protein or mRNA; 20 μg of total RNA was used in each reaction. The 447-nt hybridization probe in this experiment was prepared from a wild-type su(s) cDNA clone and spans the region that encodes the ARMs (see Materials and Methods). The su(s)wt and su(s)ΔARM1,2 protected fragments are 394 and 262 nt, respectively. Indicated beneath the lanes are the relative RNA levels. The values shown are the average of three separate experiments performed with the same RNA preparations. The standard deviations were less than 10% of the values shown.