TABLE 1.

Viability of flies ectopically expressing UAS-su(s)wt under control of different GAL4 drivers^a

Line	GAL4		No. of GAL4/UAS-su(s)wt lines tested	% Viability
	Driver	Expression pattern		Mean	Range
e22c	e22c-GAL4	Ubiquitous	13	0	0
2023	sev-GAL4	Restricted	15	82	38–96
1799b	hs-GAL4	Restricted	5	100	100
1878	T80-GAL4	Ubiquitous	10	10	0–90
1774	69B-GAL4	Restricted	14	32	0–100
1795	30A-GAL4	Restricted	6	91	76–104

^aTransformant lines, homozygous for a UAS-su(s)wt transgene, were crossed to GAL4 driver stocks, and the progeny were reared at 25°C. Multiple transformant lines were tested, and a single vial cross was set up for each transformant line. Viability was determined by calculating the percentage of progeny recovered that carried both transgenes relative to the expected number. Expression of the different GAL4 drivers (Bloomington Stock Center designations) has been characterized primarily in embryos and larval imaginal discs. The terms “ubiquitous” and “restricted” refer to the GAL4 expression pattern at the developmental stages or in tissues where it has been examined. 

^bThis cross was performed without heat shock induction. The 1799 stock was homozygous for the hs-GAL4 driver, whereas the other GAL4 driver stocks were heterozygous with a balancer chromosome. Thus, this cross lacks a control group of siblings, and the 100% value in this case is an estimate of viability (see Materials and Methods).