TABLE 2.
su(s) transgene | Transformant line |
sev-GAL4/UAS-su(s) progeny
|
|||
---|---|---|---|---|---|
% of total
|
% Viability
|
||||
25°C | 18°C | 25°C | 18°C | ||
None | NA | 55.9 (3) | 55.4 (2) | 100 | 100 |
wt | 18A | 33.1 (12)* | 46.4 (3)*** | 59 | 84 |
25A | 20.9 (3)**** | 30.8 (4)*** | 37 | 56 | |
27A | 10.7 (8)*** | 30.5 (5)** | 19 | 55 | |
ΔARM1 | 15B | 48.4 (5)* | 53.8 (1)* | 87 | 97 |
19C | 52.6 (1)* | 52.5 (4)* | 94 | 95 | |
28A | 35.6 (2)*** | 34.5 (4)*** | 64 | 62 | |
ΔARM2 | 1A | 33.0 (2)*** | 27.2 (5)*** | 59 | 49 |
4E | 50.1 (3)* | 27.2 (7)** | 90 | 49 | |
40C | 39.0 (10)* | 31.2 (3)**** | 70 | 56 | |
ΔARM1,2 | 9B | 49.4 (5)* | 49.4 (6)* | 88 | 89 |
10C | 54.5 (3)* | 56.7 (2)* | 97 | 102 | |
17A | 45.2 (2)** | 46.0 (7)* | 81 | 83 |
Females homozygous for a UAS-su(s) transgene on the second chromosome were crossed with heterozygous males carrying a second chromosome insertion of the sev-GAL4 transgene and a second chromosome balancer marked with Cy. The vials were incubated at either 25 or 18°C. Control crosses between yw females from the injection stock, lacking a UAS-su(s) transgene, and sev-GAL4/Cy males were performed in parallel. The percentage of sev-GAL4/UAS-su(s) progeny was determined by calculating the percentage of progeny with straight wings. Viability was measured by determining the percentage of straight-winged progeny obtained from the experimental versus the control cross. Standard deviations are shown in parentheses. P values, calculated by Student's t test, indicate the probability that the number obtained from each experimental cross is the same as number from the control cross: ∗, P > 0.05 (not significantly different from the control); ∗∗, 0.05 > P < 0.01; ∗∗∗, 0.01 > P < 0.001; ∗∗∗∗, P < 0.001. NA, not applicable.