Fig. 3.

VPS25 regulates the glioma cell cycle. A The effect of VPS25 on the cell-cycle distribution of U87MG cells was monitored via flow cytometry. The VPS25-silenced U87MG cells were arrested at the G0/G1 phase of the cell cycle. B Histograms show the percentage (%) of cell populations at different stages of the cell cycle in A. C The VPS25-silenced U251 cells were arrested at the G0/G1 phase of the cell cycle. Experiments were performed and are presented as in A. D Histograms show the percentage (%) of cell populations at different stages of the cell cycle in C. Data are mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. NC: negative control; siVPS25: VPS25 gene silencer