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. 2021 Dec 4;20:158. doi: 10.1186/s12943-021-01457-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — The biological function of circAXIN1. a The RNA and protein levels of circAXIN1 are significantly knocked down by three specific siRNAs, while the expression of linear AXIN1 is unaffected. An illustration of the junction site of circAXIN1 targeted by siRNAs is shown. b Cell proliferation is significantly repressed by circAXIN1 siRNA1 and siRNA3, in both AGS and N87 cells in the EdU assay. c Cell migration of AGS (treated with 10 μg/mL mitomycin for 1 h) and invasion of AGS and N87 cells are both inhibited by circAXIN1 siRNA transfection. d Cell migration is inhibited by circAXIN1 siRNAs in AGS cells (treated with 10 μg/mL mitomycin for 1 h) in the wound healing assay. e Colony formation ability is suppressed in AGS cells with circAXIN1 siRNA transfection. f-h Cell proliferation (f), cell invasion (g), and cell migration (g-h) are enhanced with circAXIN1 or linear AXIN1-295aa transfection in AGS and N87 cells. AGS cells are treated with 10 μg/mL mitomycin for 1 h in a migration assay, 48 h after transfection (g-h). EV, empty vector