FIG. 1.

Alignment of the sequences of the various profilin isoforms and translated cDNAs and ESTs from humans, rats, and mice. The sequences are human (Hum) profilin I (pfn1; GenBank accession no. NM_005022) (24), human profilin IIa (this work; accession no. AF228738), human profilin IIb (pfn2; accession no. NM_002628) (this work and reference 20), the translated Rat IIa+b long transcript (RatIIa, accession no. AF228736, and Rat IIa+b, accession no. AF228737), and MouseIIa+b (this work) (also derived from EST AA032658). HS101130 is from human cells, and AA942886 is a combination of two overlapping rat ESTs (AA942886 and H32106). Profilins IIa and IIb differ only in the underlined region; residues different in the IIb sequences are shaded. An asterisk denotes a stop codon, X denotes an unidentified residue, and a dash denotes a gap; the arrow indicates where the sequences diverge as a result of alternative splicing (also Fig. 3).