FIG. 7.

Antibodies G117 and G124 distinguish between profilin I and profilin II on Western blots. Decreasing amounts (as indicated) of human profilin I and bovine profilin IIa were used to test the specificity of the antibodies. Based on the intensities on these Western blots, we determined relative amounts of profilin in the lysates (Fig. 8A, B, and D) by performing the Western blottings simultaneously.