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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: J Biol Inorg Chem. 2021 Sep 27;26(8):947–955. doi: 10.1007/s00775-021-01904-5

Figure 2.

Figure 2.

X-band EPR spectra at 20 K of 0.4 mM Fe(III)ADO in the absence and presence of various substrate analogues and/or O2•− surrogates. A) Fe(III)ADO, B) Fe(III)ADO incubated with a 200-fold excess (80 mM) of azide, C) Fe(III)ADO incubated with a 5-fold excess (2 mM) of 2-AET, D) Fe(III)ADO incubated with a 5-fold excess (2 mM) of 2-AET and a 200-fold excess (80 mM) of azide, E) Fe(III)ADO incubated with a 10-fold excess (4 mM) of 3-MPA, F) Fe(III)ADO incubated with a 10-fold excess (4 mM) of 3-MPA and a 200-fold excess (80 mM) of azide, G) Fe(III)ADO incubated with a 10-fold excess (4 mM) of Cys, and H) Fe(III)ADO incubated with a 5-fold excess (2 mM) of Cys and a 200-fold excess (80 mM) of azide. Effective g values are provided for the S = 5/2 species. The hyperfine structure in the 3000–3700 cm−1 Gauss region is due to a minor Mn(II) impurity.