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. Author manuscript; available in PMC: 2021 Dec 4.
Published in final edited form as: Nat Struct Mol Biol. 2021 Oct 8;28(10):847–857. doi: 10.1038/s41594-021-00667-6

Fig. 5 |. Electron microscopy images with negative stain of K-Ras assembly.

Fig. 5 |

a–d, K-Ras wild type at a 10 μM concentration; maleimide lipids were used at the following percentages: 0% (a), 5% (b), 10% (c) and 20% (d). e, 2D class average of the K-Ras wild-type particles. f, D154Q K-Ras at a 10 μM concentration with 20% maleimide lipids. g, K88D K-Ras at a 10 μM concentration with 20% maleimide lipids. h, Workflow of the experiment. After incubation with the monolayer lipid overnight, the K-Ras particles were moved to a carbon-coated copper grid, washed intensively, negatively stained and examined by electron microscopy. Scale bars, 200 nm.