Table 2. Characteristics of primer pairs used in this study for multi-locus sequence typing (MSLT) identification of isolates and specific PCR.
| Locus/function | Primer | Primer sequence (5′–3′) | Reference | Annealing temperature (°C) | Amplicon length (pb) |
|---|---|---|---|---|---|
| Alta-1/Alternaria major allergen 1 | Alt-for | ATGCAGTTCACCACCATCGC | Hong et al. (2005) | 57 | 472 |
| Alt-rev | ACGAGGGTGAYGTAGGCGTC | ||||
| EndoPG/Endopolygalacturonase | PG3 | TACCATGGTTCTTTCCGA | Andrew, Peever & Pryor (2009) | 56 | 464 |
| PG2b | GAGAATTCRCARTCRTCYTGRTT | ||||
| OPA 10-2/Anonymous noncoding region |
OPA10-2L | TCGCAGTAAGACACATTCTACG | Andrew, Peever & Pryor (2009) | 62 | 634 |
| OPA10-2R | GATTCGCAGCAGGGAAACTA | ||||
| AMT-1/Non-ribosomal peptide synthethase | LinF1 | TATCGCCTGGCCACCTACGC | Johnson et al. (2000) | 65 | 496 |
| LinR | TGGCCACGACAACCCACATA | ||||
| AMT-2/Aldo-keto reductase | AMT2-f2 | GTTGCAGAATCGCAAACTCA | Roberts, Bischoff & Reymond (2012) | 57 | 653 |
| AMT2-r2 | GGCTCTTGGTCTCAAATCCA | ||||
| AMT-14/Unknown function | AMT14-EMR-F | TTTCTGCAACGGCGKCGCTT | Armitage et al. (2015) | ||
| AMT14-EMR-R | TGAGGAGTYAGACCRGRCGC | 66 | 436 |