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. 2021 Nov 30;16:7831–7846. doi: 10.2147/IJN.S334194

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© 2021 Wu et al.

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Characterization of sEV preserved in GelMA hydrogels. (A) sEV particle number preserved by different methods for different time, n = 3 each group. PC, positive control. (B) In vitro fluorescence image was used to evaluate the storage effects of different preservation methods for different days and (C) the fluorescence value, n = 3. (D) Average size of sEV particles preserved by different methods for different time, n = 3 each group. (E) Changes of particle size distribution of sEV preserved by different preservation methods. (F) TEM image for fresh sEV and sEV preserved by different preservation methods at 28 days. Scale bar = 100nm. (G) Western blot analysis of CD81 and CD9 in sEV preserved by different preservation methods. The significance (A, C and D) was tested with one-way ANOVA with Tukey posthoc test. (**p < 0.01, ***p < 0.001).