Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 30;16:7831–7846. doi: 10.2147/IJN.S334194

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Wu et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

The effect of preserved sEV on HUVEC proliferation, migration, tube formation and angiogenesis differentiation. (A) Recovery rate of sEV from different preservation method by TEI isolation. (B) Proliferation of HUVEC co-cultured with fresh sEV and sEV preserved in GelMA hydrogels for 28 days. n = 3 for each group. (C) Images of migrated HUVECs in different group. Scale bar = 200μm. n = 3 for each group. (D) Images of scratch assay in different group. Scale bar = 100μm. Time transition of the percentage of cell-free zone against initial scratch area after 12 hour, n = 3 for each group. (E) Tube-like structures of HUVECs in different group. Scale bar = 200 µm. n = 3 for each group. (F) The expression of angiogenesis markers (VEGF, FGF2, CD31, Angiogenin) was detected by qRT-PCR at 4 days post sEV treatment, n = 3 for each group. The significance (A–E) was tested with one-way ANOVA with Tukey posthoc test. (*p < 0.05, **p < 0.01, ***p < 0.001).