Figure 3.
EGFR inhibition impairs HUVEC functions. (a) Relative EGFR mRNA expression in control, shNC, and shEGFR HUVECs. (b) Effect of EGFR shRNA treatment on HUVEC proliferation (CCK-8 assay). (c) qRT-PCR results showing Cyclin D1 and Cyclin D3 expression in HUVECs treated with EGFR shRNA. (d) qRT-PCR results showing Bcl-2 and Bax expression in HUVECs treated with EGFR shRNA. (e, f) Transwell migration assay findings depicting the effect of EGFR shRNA on HUVEC migration. Scale bar: 100 μm.