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. Author manuscript; available in PMC: 2022 Nov 15.
Published in final edited form as: Cell. 2021 Oct 21;184(23):5759–5774.e20. doi: 10.1016/j.cell.2021.09.032
Nucleic Acids in the current study Sequences (5’−3’)
20bp dsRNA GACCUUCUGAUCAGAAGGUC
(palindromic sequence, self-annealed in Hao Wu Lab)
20bp dsDNA GACCTTCTGATCAGAAGGTC
(palindromic sequence, self-annealed in Hao Wu Lab)
20nt ssRNA AACUCAUCUCGACCUUCUGA
20nt ssDNA AACTCATCTCGACCTTCTGA
16bp dsRNA AAAUAAGCGCUUAUUU
(palindromic sequence, self-annealed in Hao Wu Lab)
Cy5–45bp-dsRNA(+) Cy5-AAACAAAAACAAAACAAACAACACAACAAACAAAACAAAAACAAA
Cy5–45bp-dsRNA(−) UUUGUUUUUGUUUUGUUUGUUGUGUUGUUUGUUUUGUUUUUG UUU
5’ 6-FAM™-dsRNA-60bp (+) 5’ 6-FAM™-AUCACACACCCAGCGCUGGACGGCCACCCAC AACCUCCCAAGGAACUCAUCUCGACCUUC
5’ 6-FAM™-dsRNA-60bp (−) GAAGGUCGAGAUGAGUUCCUUGGGAGGUUGUGGGUGGCCGUC CAGCGCUGGGUGUGUGAU