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. 2021 Dec 4;8:40. doi: 10.1186/s40580-021-00291-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Investigation of the cell behaviors on the GO-coated NMPA. a FE-SEM images of micro-nano pattern on silicon wafer and NMPA. b Raman intensity map of the LGO-, 10-sGO-, and 5-sGO-coated micropattern, nanopattern, and NMPA. c Confocal microscope images of the cells on bare PDMS, NMPA, LG-NMPA, 10-sG-NMPA, and 5-sGO-NMPA immunostained with actin (Red) and Hoechst (Blue). d The cell orientation graph of the cells on the bare PDMS, micropatterned PDMS, NMPA, LG-NMPA, 10-sG-NMPA, and 5-sGO-NMPA. e Cell alignment index of the cells on bare PDMS, micropatterned PDMS, NMPA, LG-NMPA, 10-sG-NMPA, and 5-sGO-NMPA. f−h Cell spreading area (f), circularity (g), and cell aspect ratio (h) of the cells on the bare PDMS, micropatterned PDMS, nanopatterned PDMS, NMPA, LG-NMPA, 10-sG-NMPA, and 5-sGO-NMPA. (* p ≤ 0.5, *** p ≤ 0.001)