Table 1 ∣.
Examples of MS methods used for analyzing proteins in single cells
| Sample preparation |
Cell types | No. of single cells |
Total number of proteins |
MS instruments | Ref. |
|---|---|---|---|---|---|
| Isobaric carrier | |||||
| mPOP | U937 monocytes and macrophages | 1,490 | 3,042 | Q Exactive | 18 |
| nanoPOTS | C10, RAW and SVEC cells | 61 | 1,225 | Q Exactive plus hybrid quadrupole-Orbitrap | 37 |
| nPOP | HeLa and U937 cells | 938 | 2,756 | Q Exactive | 23 |
| Label-free | |||||
| nanoPOTS | HeLa, human motor neurons (MN) and interneurons (IN) | 6 | 1,012 (MN) 1,085 (IN) |
Orbitrap Tribrid Eclipse with FAIMS | 34 |
| T-SCP | HeLa cells | 430 | 1,536 | timsTOF SCP | 35 |
| OAD | HeLa, mouse oocytes | 6 | 51 from HeLa 355 from oocyte | Orbitrap Elite | 38 |
The table summarizes examples of label-free and multiplexed (isobaric carrier) methods, highlighting the sample preparation method, total number of successfully analyzed cells, and total number of proteins quantified across many single cells; the number of proteins quantified per cell is smaller. Some studies were not included because of space limitations.