Fig. 4.

Oral N-PPG treatment induces expression of the mouse brain mitochondrial protease YME1L1. A Total RNA extracted from single control (C) and 200 mg/kg N-PPG-treated mouse brains analyzed by RTqPCR to show 1.7-fold induction of chaperone HSP-60 transcripts and 1.4-fold induction of mitochondrial protease YME1L1 transcripts relative to housekeeping transcripts (GAPDH and β2 M) following 9 days of oral N-PPG treatment. B Violin plots (median: dark line, open box: Q1–Q3, distribution with max./min. values) of PRODH and YME1L1 gene expression values from RNAseq analysis of brain samples from all four control and all four 50 mg/kg N-PPG-treated mice (p values determined by Wilcoxon test). C Western blots of whole brain protein lysates from all saline-treated control and 50 mg/kg N-PPG-treated mice, as analyzed in Fig. 3B, with bar graphs below western blots showing image analysis quantification (mean ratio values ± SD) of 80 kDa YME1L1 expression normalized to either 42 kDa β-actin or 30 kDa Rieske, indicating a near 40% increase in mean YME1L1 expression following N-PPG treatment (ANOVA F test p values)