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. 2021 May 13;23(12):2014–2027. doi: 10.1093/neuonc/noab114

Fig. 2.

Fig. 2

NF2 loss sensitized meningioma cells to Erastin-induced ferroptosis. CH157 (A–F) and IOMM-Lee (G–L) cells transduced with control (Vector) and NF2 expression/silencing lentivirus vector (Lv-NF2/sh-NF2) were stimulated with 6 µM Erastin for 24 hours to trigger ferroptosis. Fer-1 (3 µM for 24 hours) was used to inhibit ferroptosis. (A–D) Western blot assays and densitometry quantification for NF2, ACSL4, and GPX4. n = 3 experiments. GAPDH was used as a control. (E) Lipid peroxidation tested by MDA assays. n = 5 experiments. (F) Cytotoxicity tested by LDH assays. n = 5 experiments. (G–J) Western blot assays and densitometry quantification for NF2, ACSL4, and GPX4. n = 3 experiments. (K) Lipid peroxidation tested by MDA assays. n = 5 experiments. (L) Cytotoxicity tested by LDH assays. n = 5 experiments. *P < .05, **P < .01, and ***P < .001. Error bars represent SD. Abbreviations: ACSL4, acyl-CoA synthetase long-chain family member 4; GPX4, glutathione peroxidase 4; NF2, neurofibromatosis type 2.