Fig. 4.

MEF2C promoted the expression of both NF2 and E-cadherin. (A) MEF2C was screened out from top10 transcription factors regulating NF2 and E-cadherin (CDH1) expression (JASPAR 2020). (B and C) Dual-luciferase reporter gene assays for NF2 and E-cadherin promoters. MEF2C increased luciferase activity. n = 3 experiments. (D and E) Western blot and densitometry quantification for MEF2C, NF2, and E-cadherin. GAPDH was used as a control. n = 3 experiments. (F) ChIP assays. (G) Structure drawing for wild type (WT) and mutated sequences of NF2 and E-cadherin (CDH1) promoters. Luciferase reporter gene assays were used to verify MEF2C-binding sites. n = 3 experiments. **P < .01 and ***P < .001. Error bars represent SD. Abbreviations: MEF2C, myocyte enhancer factor 2C; NF2, neurofibromatosis type 2.