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. 2021 Jul 22;30(24):2469–2487. doi: 10.1093/hmg/ddab200

Figure 2 .

Figure 2

Characterization of selected clones of control and HD ISPNs. (A) Phase-contrast images of non-disease control (33Q, clone EE) and HD (180Q, clone n14) ISPNs demonstrate striatal precursors phenotypes before (undifferentiated) and neural phenotypes after differentiation (differentiated). Differentiation to MSN-like phenotype was performed for 14 days as described in Materials and Methods. Scale bar, 100 μm. (B) G-banding analysis demonstrates normal karyotype of ISPNs from representative karyotype spread images. (C) Immunofluorescence analysis of ISPNs shows expression of striatal precursor markers GSX2 and Meis2 in undifferentiated HD cells (180Q, clone n14) (upper four panels) as well as neural precursor marker Nestin in undifferentiated control (33Q, clone EE) and HD (180Q, clone n14) cells (bottom panels). Scale bars, 100 μm. (D) Immunofluorescence analysis (with MCA2051 Ab) of undifferentiated ISPNs shows expression of HTT predominantly in the cytoplasm of control (33Q, clone EE) and HD (180Q, clone n14) cells. Confocal images.