Figure 3 .

Immunophenotyping of differentiated ISPNs demonstrates MSN-like phenotype of selected clones. (A) Immunofluorescence analysis of ISPNs demonstrates expression of MAP2 (mature neuron marker) and DARPP32 (MSN marker) in differentiated ISPNs (180Q, clone n14) (lower row). No expression of the markers was observed in the undifferentiated ISPNs (upper row). (B) Co-expression of other MSN markers Ctip2 and GABA with neural markers TUJ1 and MAP2 was also observed in the differentiated ISPNs (180Q, clone n14). Nuclei stained with Hoechst. Scale bars, 100 μm.