Table 2.

Comparison of HD iPSC and ISPN cell models

	HD iPSCs	ISPNs
Starting cell culture	iPSC clones or neural precursors (e.g. ‘EZ-spheres’ in suspension)	Homogeneous culture of adherent cells
Differentiation time	8 weeks	2 weeks
Heterogeneity of differentiated population	5–10% of DARPP32/Ctip2 positive cells; up to 40–50% glia	over 90% of DARPP32/Ctip2 positive cells
Expansion and modification	Complicated methods of propagation; difficult to subclone	Easy to expand adherent cell lines; relatively easy to derive lines by single-cell cloning followed by genetic or other modifications
Generation of material for biochemical characterization	Restricted amount of material after differentiation, usually requiring ICC or single-cell molecular analysis	Can generate large amounts of material applicable for biochemical analysis including Omics and Western blotting, and for screening